Modern marking techniques for amphibians, fish, and even some invertebrates like crayfish includes acrylic elastomer. Acrylic elastomer is injected just below the skin and needs to be placed where the tissue is at least semi-transparent and pulls tight against muscle tissue. Multiple colors are available so independently marking multiple individuals in a population is relatively simple. Marking endangered ranid frogs that have been reared in captivity, the Atlanta Botanical Garden's amphibian conservation program head starts Gopher Frogs (Rana capito) in collaboration with the Nature Conservancy and the Georgia Department of Natural Resources. Head starting program offer a higher percentage of survivorship through the larval stage for release into protected habitat.